Notice of the Ministry of Agriculture on Printing and Distributing the Monitoring Plan for Animal H7N9 Avian Influenza in the Second Half of 2013
In order to further improve animalsH7N9Avian influenza prevention and control work, according to the current prevention and control situation, our department organized and formulated the "2013Animals in the second half of 2008H7N9Avian Influenza Monitoring Program is hereby printed and distributed to you, please follow it.
Ministry of Agriculture
2013yeareightmoonfivesun
2013Animals in the second half of 2008H7N9Avian influenza surveillance program
I. Purpose of monitoring
In order to find and master in timeH7N9The infection of avian influenza virus in animal groups, analysis and evaluation of the mode and law of transmission, andH7N9Avian influenza virus variation trend, timely removal of animal populations.H7N9The pathogen of avian influenza provides scientific basis.
Second, the monitoring requirements
Combine "2013National Animal Disease Monitoring and Epidemiological Investigation Plan (Agricultural Medical Development [2013〕nine) on avian influenza monitoring work arrangements, to carry out the second half of the animal.H7N9Avian influenza surveillance.
Third, the monitoring object
Chickens (laying hens, yellow-feathered broilers and other chickens with a long feeding cycle), waterfowl (ducks and geese), artificially raised pigeons and quails, wild birds, and the environment of relevant high-risk areas.
IV. Monitoring Scope and Time
(1) Monitoring scope
oneAll live poultry wholesale markets;
2Live poultry trading stalls (stalls) in farmers’ markets, large-scale poultry farms, free-range households, poultry slaughterhouses and migratory bird habitats.
(2) Monitoring time
All provinces and national reference laboratories should combine the monitoring of avian influenza in the second half of the year.nineConduct a centralized monitoring in January.
V. Monitoring quantity
Live poultry trading market by2%The expected prevalence rate was randomly sampled and monitored, and double swab samples and serological samples from throat and cloaca were collected in parallel.150Copies, environmental samplesthirtyFor testing.
Check outH7N9In the live poultry trading market with positive samples of avian influenza, epidemiological investigation should be conducted in time, and key investigations should be carried out on related farms, and serological and pathogenic samples should be collected from each farm.thirtyShare.
The sampling quantity of other places is in accordance with "2013National Animal Disease Monitoring and Epidemiological Investigation Plan (Agricultural Medical Development [2013〕nineNo. execution).
Sixth, the detection method
(A) serological detection methods
Hemagglutination inhibition test (HI), detection of serumH7Hemagglutinin antibody of subtype avian influenza virus. Refer to Diagnostic Techniques for Highly Pathogenic Avian Influenza (GB/T 18936-2003）。
(2) Pathogen detection methods
The fluorescence quantification recommended by the state was adopted.RT-PCRDetection method: throat and cloaca swab samples are detected.H7Subtype avian influenza virusHAGene fragment. Refer to the instructions for the recommended reagent (box) for specific operation.
VII. Criteria for determination
oneAntibody positive:According to the Diagnostic Technique of Highly Pathogenic Avian Influenza (GB/T 18936-2003), through hemagglutination inhibition test.HITesting,HIAntibody level ≥2fourThe result was positive.
2Pathogenic monitoring is suspected to be positive:The fluorescence quantification recommended by the state was adopted.RT-PCRDetection method, the result is positive.
three. Positive diagnosis:Individuals suspected to be positive in pathogen monitoring were diagnosed by the National Avian Influenza Reference Laboratory and the results were positive.
four. Positive population:At least detectoneA population of confirmed positive individuals.
VIII. Other matters
(a) monitoring results reported in a timely manner.
Local animal disease prevention and control institutions at all levels will, through the animal health monitoring information system of the National Animal Health Monitoring Information Platform,H7N9Avian influenza monitoring results are reported to China Animal Disease Prevention and Control Center step by step.
If positive individuals are detected, they should be reported to China Animal Disease Prevention and Control Center in time, and the positive samples should be sent to the National Avian Influenza Reference Laboratory for diagnosis; If the diagnosis is positive, the national reference laboratory should complete the analysis and identification of the virus as soon as possible, and report the results to the Veterinary Bureau of the Ministry of Agriculture in a timely manner, and send a copy to China Animal Disease Prevention and Control Center and relevant provinces.
(2) Strict disposal
For the positive group, in strict accordance with the "animals"H7N9Guidelines for Emergency Disposal of Avian Influenza (Trial).
(3) developing animalsH7N9Epidemiological investigation of avian influenza
Once there is human infection,H7N9Bird flu cases, all localities should promptly carry out animal.H7N9Epidemiological investigation of avian influenza.
(D) to carry out animalH7N9Avian influenza related research
National Reference Laboratory of Avian Influenza Should Develop AnimalsH7N9The biological characteristics of avian influenza pathogens and other related research, when necessary, the professional laboratory of avian influenza with relevant qualifications can participate in the relevant detection, diagnosis and research work.
(5) Do a good job in data recording and preservation.
All localities should strictly abide by the relevant management system of monitoring and sampling, and fill in the sampling record form in a standardized way to ensure that the record is true, accurate and traceable.
(6) Strengthen personnel protection
In sampling and monitoring, it is necessary to strengthen personnel protection and strictly implement the Technical Specification for Personnel Protection of Highly Pathogenic Avian Influenza.